Comportamiento de trichoderma sp. Bajo diferentes condiciones de laboratorio

Abstract

This research was conducted in order to determine the behavior of Trichoderma sp at laboratory level, through the collection, identification, isolation and purification to different conditions of temperature and culture media used as substrates for growth and development. This test was conducted in the laboratory of Plant Protection of the Faculty of Agricultural Sciences, Technical University of Ambato, the experimental design of randomized blocks with factorial added (2x3x2), being two types of races or strains of Trichoderma was used: harzianum and Minutisporium, which were exposed to three media such as agar-potato-dextrose culture (APD), Murashige & Skoog and Czapek, and two temperatures of 13.5 °C and 27 °C, this three replications. Later analysis of variance (ANOVA) with the respective tests of significance of 5% Tukey was performed to determine the best treatment research. The best results were obtained at 5 days after sowing of Trichoderma harzianum and Trichoderma minutisporium, middle Czapeck because of its high nutritional content that facilitated the development of fungus mycelium and proliferation of spores at a constant temperature of 27 °C. Regarding the growth area having a development we 6.82 cm2 per day. Characterized species during this investigation bring great benefits to farmers as they demonstrate antagonistic action of plant pathogenic fungi living in the soil and bring serious crop losses. That is why this research through a manual for the artisan production of Trichoderma harzianum and Trichoderma minutisporium, suitable for agricultural use of a simple, economical and efficient way estabece. Keywords: Trichoderma, native strains, colony forming units culture media