Desarrollo de un protocolo de establecimiento in vitro de mora de castilla (Rubus glaucus Benth) a partir de meristemos axilares

Abstract

The castilla blackberry (Rubus glaucus Benth) has become one of the agricultural products of importance in the country due to its nutritional, medicinal properties as its economic potential, which has allowed having great acceptance in both national and international market. However, its vegetative propagation has been affected by diseases, producing loss of the fruit quality. Plant tissue culture is offered as an alternative to these issues. The aim of this research was to develop an in vitro protocol for the establishment of castilla blackberry from axillary meristems. For this, two disinfection protocols were proved; method A (10 minutes 30 percent sodium hypochlorite, alcohol 70 percent for 1 minute) and the method B (sodium hypochlorite 20 percent for 12 minutes; 70 percent for 2 minute alcohol). For establishment of the meristems, two mediums were developed; medium 1 (1 mg/L BAP; AG3 0.03 mg/L; pH 5.3) and medium 2 (BAP 1.5 mg/L; AG3 0.03 mg/L; pH 5.8), A control treatment was done for each experiment. As results, 89, 33 percent of explant viability was achieved using method A of disinfection. Using medium 2, an average growth of 0.7 more less 0.1cm was produced, with large and vigorous leaves.