Estandarización del protocolo de micropropagación in vitro de Eryngium (eryngium maritimum) a partir de meristemos axilares en la empresa LePlant

Abstract

To obtain stable and quality plants for agricultural crops, it is necessary to establish a micropropagation protocol that ensures these characteristics so that the farmer can trust his harvest, so the importance of this method lies in the characteristics of its products. In this work the evaluation of parameters was carried out in the micropropagation stages of Erygium maritimum obtained in the floriculture FLOWER VILLAGE CIA. LTDA, the evaluation consisted in three disinfection treatments with sodium hypochlorite, two introductory treatments with variation in the concentration of benzylaminopurine, three multiplication treatments varying the concentration of the Murashige & Skoog medium (MS) and three rooting treatments varying the concentration of indole butyric acid and naphthalene acetic acid, this was plant growth hormones, in each stage the treatment that gave the best performance in obtaining shoots was chosen through analysis of variance and marginal means. Once the formulations of the medium were obtained in each stage, a micropropagation protocol was written, despite maintaining asepsis and control in each stage, vitrification was obtained in the multiplication, which is why a large part of the individuals who started in the process. But it was possible to obtain viable and contamination-free organisms that can be used for acclimatization and planting in the field, to generate plantations of this ornamental plant and expand its production and export, in addition, the field is open for similar studies with other plant species and to give added value to the native Ecuadorian plants.