Efecto in vitro de Valeriana rígida y Valeriana Decussata en la apoptosis tardía en células cancerígenas

Abstract

Cancer represents a public health problem worldwide, especially breast cancer, which occupies the first place of incidence in the country, followed by thyroid cancer. Ecuador has a very biodiverse territory with an extensive variety of medicinal plants that offer a possible therapeutic potential; however, there are few studies that validate these properties. Reason enough to contemplate a number of possibilities in the molecular and biochemical study of different types of plants, in particular of the valerian ones. The objective of this project was to evaluate the expression of the p53 gene by means of qRT-PCR of the Valeriana rigid and Decussata extracts in the MCF7 cell line, in addition to late apoptosis. The results showed that the relative expression of the pro-apoptotic gene p53 in relation to the control showed that the treatments; Valerian Decussata root and Decussata leaf stem produce increased expression of the p53 gene. On the contrary, for Valerian Rigida root and Rigida leaf stem showed a lower expression than that of the control. Regarding the late apoptosis tests using APO / bdru Tunel for control, MCF7 cancer cells were evidenced, with fragmented nuclei. For the treatment with Valeriana Decussata Root, cell fragments and other DNA fragments were observed that may show the end of the apoptotic pathway, as Valeriana Decussata leaf stem only one apoptotic cell was observed, while for rigid Valerian leaf stem and root no evidence of late apoptosis was observed.