Aplicación de fotometría reflectante y microscopia digital en el diagnóstico de infecciones de vías urinarias

Abstract

Antimicrobial resistance is a growing global problem, which also affects the common etiological agents of urinary tract infections (UTIs) and may involve increased mortality without proper treatment. There are different resistances at the outpatient versus nosocomial level that must be taken into account, any use of anti-biotics causes selective pressure on the microbiome by eliminating susceptible bacteria and increases the risk of infections by resistant bacteria. Therefore, a study was carried out at the Calderón General Hospital in the city of Quito, where 684 urine samples from outpatient, emergency and internal medicine patients were analyzed, in which Urine Culture was requested to correlate the parameters of EMO with Urine Culture. The EMO was performed in the Laura XL team, the chemical analysis, and digital microscopy, the chemical in an automated way in the Laura XL team, where the parameters of leukocytes and nitrites were taken into account for the microscopic analysis the values of leukocytes and bacteria were collected. For the urine culture we isolated the colonies, made a quantitative count and identified the most common uropathogens in patients, making a presumptive recognition. In the samples analyzed, it was found that 63% of requests for urine culture correspond to women, and it was shown that the diagnosis of UTI occurs more frequently in adult women as geriatrics. Of the samples analyzed, the positive urine cultures were 29%, in which the most frequent bacterium was E. coli.