Efecto del plasma seminal y vitamina E sobre el proceso de crioconservación y calidad post descongelado de semen ovino

Abstract

In this research work, the effect of seminal plasma and vitamin E on the post-thaw quality of ovine semen was evaluated using three treatments: T0: control treatment where a commercial extender was used, T1: vitamin E (25%) was added to the commercial extender, T2: vitamin E (25%) plus seminal plasma (50%) was added to the commercial extender. We worked with 3 rams and 1 ewe, evaluating a total of 210 sheep straws. Using a completely randomized design with 7 replicates per treatment, the results were compared by Tukey's statistical test with a significance level of 95% using the statistical program Infostat. The following variables were analyzed in two phases: for fresh semen, an individual evaluation per ejaculate/carrier was performed, consisting of a macroscopic study (volume, color, translucency) and a microscopic evaluation using conventional techniques (concentration, mass motility, morphology, vitality). For the post-thawed semen, a pool of heterospermic semen was made and aliquots were formed for each corresponding treatment, diluted, packaged, frozen and the post-thawed semen was evaluated microscopically at 0 and 2 hours by conventional techniques (concentration, individual mobility, morphology, vitality) plus osmotic resistance (HOST) and acrosome integrity. In conclusion, positive results were obtained in post-thawed semen quality by adding vitamin E and seminal plasma to the commercial diluent.