Efecto antihelmíntico in vitro de extractos de arbustivas ricas en metabolitos secundarios sobre diferentes estadios del ciclo biológico de Haemonchus contortus

Abstract

The indiscriminate use of antiparasitic drugs contributes to resistance to them. Currently, natural alternatives have been sought such as the use of secondary metabolites as antiparasitics, such as tannins that negatively affect the nutrition, movement, and reproduction of gastrointestinal nematodes in animals. The objective of this work was to evaluate the in vitro anthelmintic effect of the hydroalcoholic extract of two tannin-rich shrubs, through its application on cultures of larvae and eggs of Haemoncus contortus. The extracts of Albizia lophantha and Acacia mearnsii were applied in 4 concentrations (T0: pure extract; T1: 0.04 ml/ml; T2: 0.08 ml/ml and T3: 0.12 ml/ml) 4 repetitions of each treatment were performed and the results obtained were analyzed with the ANOVA and Tukey test in the InfoStat 2020 version program. The inhibition of hatching was evaluated as an effectiveness variable. The most effective treatments with Albizia lophantha were: T0 with a 100% inhibition and T3 with a 33% inhibition at 24h, while with Acacia mearnsii the treatments that showed the highest inhibition were T0: 100% and T3: 29.5% at 24h. As for larval survival with the Albizia lophantha extract, the most effective treatments with 0% survival were T0 at 24h and T3 at 48h, while with the Acacia meanrsii extract the T0 and T3 treatments presented 0% survival at 24 and 48h respectively.