Browsing by Author "Canseco Arrunategui, Maritza Angelica"

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    “Estudio de la expresión de genes inductores de apoptosis en línea celular mcf-7 en respuesta al tratamiento de los extractos de las plantas ilex guayusa, uncaria tomentosa y croton lechleri”.
    (Universidad Técnica de Ambato - Facultad de Ciencias de la Salud - Carrera de Laboratorio Clínico, 2019-10-01) Canseco Arrunategui, Maritza Angelica; Bustillos Ortiz PhD., Alcides Alberto
    Breast cancer is the second leading cause of death in women around the world, despite advances in medicines and therapies, it remains a public health problem, for this reason it is still necessary to investigate new active compounds that could become candidates for the development of new drugs. In this context, Ecuador is a highly diversified country with a significant number of ancestral plants to which indigenous communities attribute anti-proliferative and anti-carcinogenic properties. The objective of the research was to evaluate the effect on the expression of the Bax and bcl-2 genes by polymerase chain reaction in real time (qrt-PCR) extracts of Ilex guayusa, Uncaria tomentosa and Croton lechleri in the MCF7 breast cancer cell line. The extracts were obtained by maceration in ethanol at 96%, lyophilization and were resuspended in PBS 1X. The MFC-7 cell line was treated with the various extracts at a concentration of 3ug/ul for 24 hours. The total RNA was then extracted from the treated cells and its concentration was determined. cDNA was then synthesized and the expression of the Bax and bcl-2 genes was evaluated using a real time PCR. The obtained results showed that the ethanolic extract of Croton lechleri increased the expression of the Bax gene (p<0.05) and decreased the expression of bcl-2 (p<0.001) in relation to MCF7 cells not treated with the extract. The other two excerpts were inconclusive. The results were analyzed using variance analysis (ANOVA) in addition to a post-hoc Tukey analysis. The significance for Bax was <0.0002 and <0.0001 for bcl-2 between treatments, which is why the alternate hypothesis was accepted.