Browsing by Author "Catagña Caiza, Gloria Beatriz"

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    Concentración sérica de progesterona (P4) en ovejas sometidas a diferentes dosis de eCG - PMSG con un protocolo de IATF en la región andina del Ecuador.
    (2024-08) Catagña Caiza, Gloria Beatriz; Aragadvay Yungan, Ramon Gonzalo
    Progesterone (P4) levels increase when starting synchronization treatment with good ovarian status, which allows obtaining better-quality oocytes and increasing fertility. The objective of this study was to measure serum P4 concentrations in sheep from the Andean Region of Ecuador upon removal of the sponge, at the timeof heat detection (CD), fixed-time insemination (FTAI), and on day 7 after IATF. Located in the Andes Mountains, in the province of Pichincha, Pedro Moncayocanton, Malchinguí parish, the Huayrapungo Farm is situated at an altitude of 2600 to2800 meters above sea level and has a temperature of 7 to 17 °C. Multiparous sheep in production were of breed (Merino x Suffolk) f1, with body condition greater than 2(scale 1–5), less than 90 days open, and an age of 2–4 years. A conventional protocol divided them into two treatments: T1 (400 IU PMCG per 10 sheep) and T2 (500 IU PMCG per 10 sheep). On Day 0, of 60 mg P4 (PROGESPON,Zoetis) was administered; on Day 10, T1=400 IU was applied; and T2=500 IU of PMCG i.m. (NOVORMON-PMCG® 5000, Zoetis, Ecuador), together with 250 μg of Cloprostenol (PGF2α, Ciclase DL, Zoetis, Ecuador); on Day 12, the IVD was removed; on Day 13, DC; on Day 14, FTAI by laparoscopy; and on Day 7, after FTAI,blood samples were taken in each of these phases. On day 35, pregnancy was confirmed through ultrasonography (Minitube Vet). A generalized linear mixed model was used to analyze the data; treatment and progesterone were used as continuous variables, and pregnancy as discrete variables. T1 and T2 did not show significant differences in the pregnancy rate (60%) (P = 0.073). As well as the removal of the sponge, DC, and FTAI (P = 0.49). However, a significant difference was found on day 7after FTAI (P = 0.020*). However, serum P4 concentrations did not show any difference in the reproductive status of ewes (empty or pregnant) (P = 0.35). Animals were found to gradually increase circulating P4 concentrations after ovulation, beginning on day one and reaching the mean P4 concentration by day seven (6.9 ng/mL) during pregnancy. 500 IU PMCG significantly increased serum P4 concentrations at the beginning of the luteal phase but did not improve the pregnancyrate in female sheep.