Browsing by Author "Coba Mejía, Nelson Xavier"

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    “Determinación de Salmonella Typhi y su relación a enfermedades diarreicas en los comerciantes del mercado mayorista de la cuidad de Ambato”
    (Universidad Técnica de Ambato - Facultad de Ciencias de la Salud - Carrera de Laboratorio Clínico, 2019-05-01) Coba Mejía, Nelson Xavier; Guangasig Toapanta, Víctor HernánBqf. Mg.
    Gastrointestinal diseases are one of the main public health problems in Ecuador. They are transmitted, either by fecal-oral route, or by the consumption of contaminated water or food. Its incidence as its prevalence depends on the socioeconomic level of the patients and mainly affects the child population. The pathogens involved are viruses, parasites and bacteria. The identification of these, in clinical laboratories, focuses on clinical pathogens such as: Salmonella, Shigella, Escherichia, Vibrio, Campylobacter and Yersinia. The objective of the present study was to identify S. Typhi, as a causative agent of gastrointestinal infections in wholesale market traders, by means of the Rapid S. Typhi Antigen Test Card immunochromatography test, to verify by biochemical tests the specificity of the test and to establish the profiles of sensitivity and / or resistance presented by the bacterium isolated by antibiogram by the Kirby-Bauer method. Fifty patients were surveyed and the test was performed with Rapid S. Typhi Antigen Test Card immunochromatography in search of S. Typhi. 20% of the samples tested positive for S. typhi. The biochemical tests performed in the media TSI, SIM, Urea, Citrate, Malonate, RM-VP gave 100% positivity for S. Typhi. The antibiogram reflex resistance of 20% of the strains to Ampicillin and 100% sensitivity to Ceftazidime, Amoxicillin + Ac. Clavulanic, Trimethoprim / sulfamethoxazole, Tetracycline, Gentamycin, Ac. Nalidixico and Ceftriaxone. It was established that of the 50 cases of patients with diarrheic processes, only 20% was due to S. Typhi bacteria.