Browsing by Author "López Estrella, María Teresa"

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    Implementación del uso de Placas RODAC para el diagnóstico de micosis superficiales
    (Universidad Técnica de Ambato/Facultad de Ciencias de la Salud/Centro de Posgrados, 2022-09-01) López Estrella, María Teresa; Tinajero Vásconez, María Fernanda MsC.
    In pathologies such as superficial mycosis, it is necessary to identify the pathogen, which conventionally present as scaly lesions on the skin, a scalpel scraping is performed to observe viable fungal elements. The use of RODAC plates, a solid medium with a convex surface, with a pressure level on the lesion to be investigated, gives us the possibility of adhering a large number of microorganisms with greater repeatability and recovery, avoiding contamination when taking samples. Objective To implement the use of RODAC Plates for the diagnosis of fungi cause dermatomycosis. Methodology The present study is of a quasi-experimental type of intervention since it will analyze the taking of samples, the direct examination with KOH for the initial observation and the evaluation of development of fungi on RODAC plates. Results Of the total of 89 people analyzed, the presence of Trichophyton spp. which represents 73% while 9% corresponds to Epidermophyton spp. 18% corresponds to the absence of fungal growth, the samples were taken from the skin. The prevalence obtained is 82% with a 95% CI between 55.1 – 74.9%. In the study, the results in the fresh examination obtained 78.7% of positive cases and 21.3% represents negative samples. In the RODAC plate, development was shown in the culture medium with 80.9%, while 19.1% did not show development in the medium. Regarding sex, the majority of the participants were men represented with 62.9%, only 37.1% is assigned to women. Conclusions When implementing the use of RODAC Plates for the identification of fungi that produce dermatomycosis, infections that observe the epidermis and skin annexes, where the main characteristic skin is the affection of the keratinized superficial layers of the population that attends the center of Type C health in the city of Latacunga, it was possible to establish a procedure for taking samples directly from the lesion, obtaining fungal cultures for later identification.