Browsing by Author "Masaquiza Moyulema Rosa Angelica"

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    Determinación del perfil de resistencia antibiótica e identificación de los principales agentes patógenos causantes de mastitis clínica y subclínica en vacas lecheras
    (2025-02) Masaquiza Moyulema Rosa Angelica; Ulloa Ulloa Lourdes Anita; Ulloa Ulloa Lourdes Anita
    Mastitis is a problem that affects dairy cattle, so this disease is an inflammation of the mammary gland that can be caused by several factors. The objective of this research is to determine the antibiotic resistance profile and identify the main pathogens causing clinical and subclinical mastitis in dairy cows. Twenty-six milk samples from the Unión de Organizaciones Indígenas de Pasa (UOCAIP) were analyzed in three selective culture media such as Columbia Agar Base, salted Mannitol Agar and MacConkey Agar and by means of different biochemical tests such as catalase, coagulase and TSI the presence of Staphylococcus aureus was identified in 64.15%, coagulase negative Staphylococcus in 24.53%, Streptococcus spp. 9.43% and Escherichia coli. 1.89%. In the antibiotic resistance profile, Staphylococcus aureus showed a resistance to oxacillin and azithromycin of 88.89% and to penicillin of 83.33%; however, it was sensitive to sulfa trimethoprim of 100%, to ciprofloxacin of 94.44% and tetracycline of 88.89%. Coagulase-negative Staphylococcus presented a resistance to oxacillin of 100% and to penicillin of 62.5%, while it was sensitive to sulfa trimethoprim, ciprofloxacin and azithromycin of 100%. Streptococcus spp. showed 100% resistance to ciprofloxacin, intermediate sensitivity to tetracycline and azithromycin of 20% and sensitivity to sulfa trimethoprim and penicillin of 100%, to tetracycline 60% and to azithromycin 80%. Finally, E. coli showed a resistance to penicillin and azithromycin of 3.85%, intermediate sensitivity to azithromycin and ciprofloxacin of 3.85%, and sensitivity to sulfathrimethoprim and tetracycline of 3.85%.