Browsing by Author "Pazmiño Eugenio, Damaris Abigail"

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    Evaluación de diferentes combinaciones de polímeros en la microencapsulación de licopeno de tomate de árbol (Solanum betaceum)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2019-12) Pazmiño Eugenio, Damaris Abigail; Fernández Rivero, Danae
    The concentrate of lycopene of tree tomato (Solanum betaceum) was microencapsulated using four differents combinations whose were evaluated through the antioxidant capacity. The combinations consisted of varying the load, with respect to the polymers used that were maltodextrin and gum arabic with modifications in the entry and exit temperature in the microencapsulation. The evaluation of antioxidant activity in vitro was developed using the DPPH test. On the other hand, Saccharomyces cerevisiae was used as a cellular model to analyze the activity in vivo comparing the growth rate it presents against high and low stress levels with hydrogen peroxide and sodium hypochlorite. It was determined that the microencapsulated with 30% lycopene, 35 percent gum arabic and 35 percent maltodextrin at an inlet temperature (Te) of 120 Celsius degrees and outlet (Ts) of 80 Celsius degrees showed greater antioxidant capacity when using the DPPH free radical method obtaining 81,84 percent inhibition equivalent to trolox concentration of 333,78 more less 4,53 µmol of trolox for gram sample, also recorded a percentage of microencapsulation efficiency of 64,42 percent, which was comprobated with the analysis in the far infrared spectrum taking into account that 83,76 percent transmittance was obtained. The antioxidant activity in vivo corroborated the previous results, because when using the microencapsulation at a concentration of 700 mg/mL, Saccharomyces cervisiae showed a growth rate greater than 1 in the presence of NaOCl and H2O2 exceeding the antioxidant capacity of vitamin C.