Browsing by Author "Proaño Mena, Dayana Alejandra"

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    Congelación de semen porcino de raza Pietrain centrifugado mediante el uso de glicerol y yema de huevo como crioprotectores
    (2024-08) Proaño Mena, Dayana Alejandra; Aragadvay Yungan, Ramón Gonzalo
    In the present investigation, the corresponding evaluation of two cryoprotectants was made: egg yolk and glycerol at different concentration percentages, used with centrifuged semen from Pietrain pigs for both the freezing and refrigeration processes in two treatments and to see their influence. in seminal quality. Frozen T1: 3.5ml of egg yolk + 1.5ml of glycerol were added. Frozen T2: 4.5ml of egg yolk + 2.5ml of glycerol were added. Which in practice corresponded to the following quantities: Refrigerated T1: 70ul of egg yolk + 30ul of glycerol were added. Refrigerated T2: 90ul of egg yolk + 50ul of glycerol were added. Two Pietrain breed stallions were used where 6 ejaculates were extracted and divided into 3 treatments, giving us a total of 18 analyzed samples. The two experimental groups were subjected to a T student comparison with a significance level of 0.05%. The corresponding analyzes were carried out divided into fresh semen and semen applied to the treatments. Where, for both, the quality was evaluated with macroscopic tests (volume, color, consistency) and microscopic tests (mass and individual mobility, normal morphology and abnormalities in the head and tail). Obtaining as a result that all the variables did not have significant differences, however, in the abnormalities section, in loose head at hour 27 after refrigerated treatment 2 a significant difference of 0.007 was obtained and at hour 0 in elongated head 0.01 was obtained. that these values are less than the established P value, which is 0.05.