Browsing by Author "Reinoso Torres, Daniel Patricio"
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Item Evaluación de cepas bacterianas resistentes a metales pesados en la zona del canal de riego Latacunga-Salcedo-Ambato con potencial biorremediador(2016) Reinoso Torres, Daniel Patricio; Ibarra López, ElizabethThe Latacunga-Salcedo-Ambato irrigation canal is supplied with water from the Cutuchi river, which irrigates important crop areas of the provinces of Cotopaxi and Tungurahua. The direct dumping of contaminated water to the river by tanneries, textile industries, sewage, slaughterhouses, factories, hospitals and agricultural activities, make this ecosystem a space susceptible to contamination by heavy metals. This study determined the resistance capacity of bacterial strains to heavy metals copper and cadmium using biomass of bacteria previously isolated from the Latacunga-Salcedo-Ambato irrigation canal. Ten bacteria from the microorganism bank of the Faculty of Agricultural Sciences were chosen at random, which are coded as BUTA1 to BUTA40 and the macroscopic and microscopic characterization was performed. The determination of bacterial resistance was analyzed by the method of bacterial growth in solid medium and liquid medium according to Serrano, 2015. The bacteria BUTA13, BUTA15, BUTA16 and BUTA19 presented a wide range of resistance to the presence of 2mM of Cu. As for the bacteria BUTA13, BUTA16 and BUTA19 presented a wide range of resistance to the presence of 2mM of Cd. The results obtained show bacterial strains isolated from a source of natural environmental contamination with potential for resistance to heavy metals constituting bacteria suitable for application in bioremediation processes of ecosystems contaminated with heavy metals such as Cu and Cd. Key words: Resistance, heavy metals, bacterial strains, bioremediation.Item Influencia de la cobertura vegetal y relieve sobre el almacenamiento de carbono en el suelo del ecosistema páramo(2020-10) Reinoso Torres, Daniel Patricio; Pomboza Tamaquiza, Pedro PabloThe investigation was carried out in the paramo of the community of Tamboloma belonging to the parish of Pilahuín in the canton of Ambato, the primary information was collected through a personal interview with the president of the community of Tamboloma, Mr. Humberto Matiag. Also, 48 soil samples were analyzed to determine the amount of soil organic carbon (COS) based on the study factors: relief, vegetation cover, depth and the variables evaluated were: COS, apparent density, pH, electrical conductivity. The main objective of the research was to determine the accumulation of carbon in natural and intervened páramo ecosystems and the relationship with climatic factors. Soil carbon content using the modified method of Walkey and Black (1947). The mean SOC content in the páramo ecosystem within a natural or grassland area was 147.05 t/ha and for a human intervention or grassland area it was 162.49 t/ha, where the soil order of páramo for the province of Tungurahua corresponds to the Andosols. The mean pH value of the soil was 5.64, the apparent density was 0.81 g/cm3 and with an average electrical conductivity of 629.79 uS/cm. In addition, the apparent density variable (R2 = 0.4093) was the only variable that presented a positive response with the amount of COS in the correlation analysis, while the pH and EC variables showed an R2 value of 0.0015 and 0.0056 respectively