Browsing by Author "Romero Rosero, Luis Fernando"

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Efecto de adición de agua de coco centrifugado en diluyente comercial como potenciador de la calidad seminal en caninos post refrigerado
(2024-08) Romero Rosero, Luis Fernando; Aragadvay Yungan, Ramon Gonzalo
The objective of this research was to evaluate the effect of adding 5% and 10% centrifuged coconut water (CCA) to a commercial diluent as an enhancer of postchilled canine seminal quality. Three treatments were evaluated: T0 (semen+diluter), T1 (semen+diluent+5%ACC) and T2 (semen+diluent+10%ACC), the variables measured were macroscopic (volume, color/opacity, pH) in fresh semen, microscopic in post-chilled semen (concentration, mobility mass, individual mobility, vitality and morphology) and complementary tests: Host Test and Acrosome Integrity Test and were analyzed in three times 24, 72 and 120 hours, the extender was mixed with the semen in a proportion of 2:1, The coconut water was previously filtered through Whatman No. 4 filter paper and centrifuged and added to the mixture of the diluent and ejaculated semen obtained from the cross-breed canine under study. The results were as follows: average volume of ejaculated semen was 5.83 mL, whitish color, and pH of 6.00, sperm concentration (millions of sperm/mL) between 227.00 x106- 288.83 x106, there was a significant difference in T1 at 24 hours in the variables mass mobility, individual mobility, vitality, morphology, Host Test , Acrosome integrity test with values of 66.67%, 78.33%, 82.50% and 83.33%, 70.00%, 42.50% respectively, which means that they showed the best percentages in each of these variables. Concluding that the addition of 5% of centrifuged coconut water to the extender and ejaculated semen maintains refrigerated canine semen quality up to 24 hours postcooling, unlike the variable integrity of the acrosome, which is drastically affected by the lack of use of cryoprotectants to the experiment.