Browsing by Author "Rubio Obando Gregory Matheo"

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    Detección de genes de resistencia a antibióticos betalactámicos (blaTEM y blaCTX-M) en Shigella spp. aislada de carne de pollo que se expende en la ciudad de Ambato
    (2025-02) Rubio Obando Gregory Matheo; Cruz Quintana Sandra Margarita
    Chicken meat is a widely consumed food that can act as a vehicle for the transmission of antibiotic-resistant pathogenic microorganisms. In this context, the main objective of this research was to determine the presence of the beta-lactam resistance genes blaTEM and blaCTX-M in Shigella spp. strains isolated from chicken meat sold in the city of Ambato. 17 samples of chicken meat were collected from slaughterhouses (9 samples) and informal sales points (8 samples), and phenotypic and molecular identification of the strains was performed. The Shigella species identified were S. sonnei (58.82%), S. flexneri (29.41%) and S. dysenteriae (11.77%). The blaTEM gene was detected in 23.53% of the samples analyzed, while blaCTX-M was present in 88.23%. Both resistant strains were found in S. sonnei and S. dysenteriae, but not in S. flexneri. The highest presence of these genes was found in samples from unregistered sales points, suggesting that inadequate hygienic-sanitary conditions contribute to the spread of resistant bacteria. The results obtained partially agree with previous studies carried out in other regions of Ecuador and Latin America, where the S. flexneri species has historically been the most prevalent. However, a change in epidemiology is observed, with a predominance of S. sonnei in the samples analyzed, which could be explained by the capacity of this species to acquire resistance genes more easily. This study highlights the importance of implementing sanitary control and surveillance measures in the chicken meat production and marketing chain, in order to prevent the spread of bacteria resistant to beta-lactam antibiotics and reduce the associated risks to public health.