Browsing by Author "Torres Valdez, Lissette Estefanía"

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    EVALUACIÓN DE LA CONFIABILIDAD DE LA METODOLOGÍA MANUAL FRENTE A LA METODOLOGÍA AUTOMATIZADA EN LA IDENTIFICACIÓN DE CÁNDIDA ALBICANS
    (Universidad Técnica de Ambato / Facultad de Ciencias de Salud / Centro de Posgrados, 2024-10-01) Torres Valdez, Lissette Estefanía; Guangasig Toapanta, Víctor Hernán
    Candida albicans is the main species related to most superficial and deep infections caused by yeasts, therefore it is necessary to have tests that are fast and accurate for timely microbiological identification through the reliability of both manual methodologies and automated methodologies such as the VITEK system and thus establish which is the best option to use in the laboratory, THE objective was to evaluate the reliability of manual methodology versus automated methodology in the identification of Candida albicans. The methodology used was a cross-sectional observational analytical study, with a quantitative approach since data collection was used based on numerical measurement and statistical analysis, it is non-experimental, according to the chronology the research is prospective, the inclusion and exclusion criteria were analyzed. The results were as follows: from 100 microbiological samples of fungi where 82% (n=82) corresponds to isolates of the albicans species, 18% corresponds to other species, which are: tropicalis (n=10), glabrata (n=5) and parapsilosis (n=3), was isolated in 82 samples of the microorganism to be identified of which it is observed that 73. 17% were isolated from vaginal secretion samples, 10.98% from skin secretions, 8.54% from seminal secretions, and 3.66% from oral and intestinal secretions. Of the total of 82 positive samples for Candida albicans, the conventional method (CHROMagar) and the automated method (VITEK) gave 70 correct identifications of the yeast and one incorrect identification of the yeast. On the other hand, CHROMagar identified 3 positive results that VITEK did not and VITEK identified 8 positive results that CHROMagar failed, the Kappa statistic (κ),. κ =0.093 indicates that there is a slight concordance between the CHROMagar and VITEK methods. The results indicate that there is no statistically significant agreement between the CHROMagar and VITEK methods for identifying Candida albicans. The odds ratios suggest some level of association, based on the Kappa statistic, an acceptable to moderate agreement between the two methods is proposed..
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    “Indice de masa corporal (imc) como factor de riesgo de insulinorresistencia en niños y adolescentes con sobrepeso y obesidad”
    (Universidad Técnica de Ambato - Facultad de Ciencias de la Salud - Carrera de Laboratorio Clínico, 2019-01-01) Torres Valdez, Lissette Estefanía; Viteri Robayo, Carmen Patricia Ing. Mg.
    One of the main health problems nowadays is obesity, it is defined as the increase of fat in a general or specific area which manifests itself in a high weight value when it is compared to other people of the same age and gender. Obesity is determined by a relationship between weight and height obtaining the body mass index. (BMI). The insulin-resistance is a health problem that is also related to obesity and overweight. Due to the fact that insulin cannot produce an appropriate biological response to the cells. Causing a decrease of its action in the metabolic control of glucose after eating a meal. The objective of this investigation is to analyze the relationship between the mass body index (MBI) and the insulin-resistance in children and teenagers with overweight and obesity in the city of Ambato. This study was descriptive, cross-sectional, with a qualitative and quantitative, character there were 60 participants, 31 were women (51,7%) and 29 men (48.3%). They were between the ages of 5 to 19 years old. The results through the MBI showed that 20 people had a normal nutritional status; however, 17 had obesity and 23 overweight. In order to determine the serum values of glucose and insulin, blood tests were made and they showed insulin-resistance by applying the HOMAR-IR formula. The majority of the participants obtained normal serum value of glucose and insulin; and however 30 % of the participants were found to be insulin resistant. The results revealed that high intakes of fat, carbohydrates and little physical activity, as well as, sedentism directly influences the nutritional status of children and teenagers. Nevertheless, there was not a significant relationship between the nutritional status of obesity and overweight with the resistance of insulin in the pediatric population of this study. Thus, the null hypothesis that states ‘The MBI is not a risk factor to develop insulin resistance in children and teenagers with overweight and obesity in the city of Ambato’ is accepted.