Browsing by Author "Trujillo Ruiz, John Michael"

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    ACTIVIDAD ANTIFÚNGICA DE ACEITES ESENCIALES EN CANDIDA ALBICANS ATCC 10231
    (2025-02-25) Trujillo Ruiz, John Michael; Proaño Pérez, María Elizabeth
    Candida albicans is a yeast located in the mouth, vagina, intestine, throat, and skin, which can proliferate and generate infections when people have a weakened immune system (1). This yeast generates biofilms, giving it a virulence mechanism and resistance to drugs such as amphotericin B or fluconazole (2)(3)(4). To address this problem, the use of essential oils with antimicrobial properties has been chosen, enhancing this effect through microencapsulation to protect the functional and biological characteristics (5)(6). This research study rigorously examined the remarkable antifungal properties of essential oils derived from Rosmarinus officinalis (rosemary), Ocimum basilicum (basil), and Cymbopogon citratus (lemongrass) against Candida albicans ATCC 10231. The Kirby-Bauer technique was modified according to the CLSI M02 standard. (7). Antifungal activity of essential oils was observed at concentrations up to 25 mg/mL for Ocimum basilicum and Cymbopogon citratus. In comparison, Rosmarinus officinalis showed activity at concentrations up to 50 mg/mL. The antibiofilm activity of Cymbopogon citratus essential oil was assessed using crystal violet dye staining, demonstrating significant inhibition from a concentration of 50 mg/mL to 12.5 mg/mL. The statistical analysis was conducted using an ANOVA test and Tukey's and Bonferroni's multiple comparison analyses. The findings indicated that the essential oils inhibit the growth and development of biofilm in Candida albicans ATCC 10231.