Tesis Medicina Veterinaria y Zootecnia

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    Influencia de extractos vegetales y aceite de sacha inchi sobre la fermentación ruminal in vitro.
    (2018) Agualongo Chango, Angel Daniel; Barros Rodríguez, Marcos A.
    The objective of the present investigation was to evaluate the effect of the addition of vegetable extracts and oil of Sacha inchi (Plukenetia volubilis) on in vitro ruminal fermentation, to reduce the emission of greenhouse gases, according to a completely randomized design, with twenty-two treatments: Albizia lophanta (AL), Acacia hayesii (AH), Acacia melanoxylon (AM), Baba de Teobroma cacao (BC), Sacha inchi oil ( ASI), a mixture of plant extracts (MSA) and a mixture of vegetable extracts with Sacha inchi oil (MCA) at doses of 0, 1, 3, 5 μl / 0.5g MS. The production of total gas, methane, CO2, in vitro digestibility, pH and Volatile Fatty Acids (VFAs) was determined. The in vitro fermentation was carried out in the laboratory with 132 bottles of 100 ml (6 bottles per treatment including the control treatment). Gas production was performed by the technique described by Theodorou et al. (1994), which consists in placing 0.500 mg of MS of sample in glass bottles with a capacity of 100 ml. In the bottles, 60 ml of inoculum (70:30, artificial saliva: ruminal content) was incubated at 39.5 ºC in an oven. The measurement of gas pressure and volume was measured manually at the following times 3, 6, 9, 12, 18, 24, 36, 48, 60, 72, 96 hours. For in vitro digestibility, the same procedure was used, but it was only incubated until 48 hours, the pH and the AGVs were incubated until 24 hours. With respect to total gas production and production of methane gas, the diets with AL 1% and ASI 1% showed the lowest results (P <.0001) in the total production of gas 506.28 and 538.87 ml / 0.5g MS (Vfi) respectively, in the production of methane gas 54,908 and 51,312 ml / 0.5 g MS respectively, while in the production of CO2 the lowest value was obtained only for AL 1% (160.45 ml / 0.5g MS). The in vitro digestibility of DM and MO showed differences (P = 0001) between treatments, with greater digestibility observed in ASI 1% (514. 27 g / Kg MS and 505.83 g / Kg MO). The pH shows difference (P = 0001) among the treatments presenting the most optimal value ASI 1% (6.95 - 6.99). In conclusion, the addition of oil of Sacha inchi (Plukenetia volubilis) 1% and extract of Albizia lophanta 1%, improves the digestibility, stabilizes the pH and reduces the emission of greenhouse gases.