Tesis Medicina Veterinaria y Zootecnia

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    Efecto antimicrobiano in vitro del aceite esencial de orégano (Origanum vulgare) sobre cepas certificadas de Escherichia coli Y Staphylococcus aureus
    (2018) Lopez Rivera, Edwin Andrés; Montero, Mayra
    The objective of the research project was to evaluate in vitro the antimicrobial effect of oil of oregano (Origanum vulgare) on strains of Escherichia coli and Staphylococcus aureus subsp. aureus, in which concentrations of 30%, 60% and 90% were evaluated in dilution of oregano plus tween 80 oil. The Minimum Inhibitory Concentration was determined for the Escherichia coli strain, where turbidity was present at 30% in all its established hours, while at 60% and 90% it did not present turbidity, on the other hand, for the strain Staphylococcus aureus, no turbidity was present in any of its concentrations; The Bactericidal Minimum Concentration was also determined on the strain Escherichia coli and Staphylococcus aureus, where the 30% Escherichia coli strain presented colony formation at all established times, while at 60% and 90% there was no bacterial growth; On the other hand, for the strain Staphylococcus aureus, there was no bacterial growth in any of the three concentrations. Regarding the sensitivity halos in the Escherichia coli strain, a minimum diameter of 13.01mm at 30% and a maximum value of 17.62mm at 60% was obtained, determining that the oil of oregano prevents the growth of bacteria from its minimum value at 30%, on the other hand, in the Staphylococcus aureus strain, a minimum value of 13.25mm was obtained at a concentration of 30% and a maximum value of 25mm at 90%. In the analysis of variance the results obtained in the treatments at 60% and 90%, are not significantly different (p = <0.05), being statistically equal with values of 17.62 mm and 16.05 mm respectively, in relation to 30% with a value of 13.01 mm in halos of inhibition for the strain Escherichia coli, while the dilutions of the treatments at 30%, 60% and 90% against the strain Staphylococcus aureus are statistically different (p = <0.05) with values of 13.25 mm, 16.35 mm and 25.00mm of inhibition halos.