Maestría en Laboratorio Clínico Mención Microbiología Clínica

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    ANÁLISIS DE LA SUSCEPTIBILIDAD A ANTIFÚNGICOS Y EVALUACIÓN DE FACTORES DE RIESGO ASOCIADOS EN CEPAS DE CANDIDA SPP. AISLADAS DE SECRECIONES VAGINALES EN MUJERES DE 22 A 65 AÑOS
    (Universidad Técnica de Ambato / Facultad de Ciencias de Salud / Centro de Posgrados, 2024-10-14) Alquinga Lovato, Jenny Carolina; Moina Veloz, Álvaro Paúl
    Candida spp. is a fungus that can be found in the vagina, causing vulvovaginal candidiasis (VC) causing gynecological problems in different ages, including those with diabetes, producing an impact on physical health, and their intimate relationships, commonly used as treatment azoles, the prognosis is good and curative, but if the predisposing factors of mycosis are not corrected a new reinfection occurs. Species identification was performed with CHROMagar candida and Biggy nickerson as control agar, in addition, antifungal susceptibility testing was performed along with a closed-question survey to identify risk factors associated with VC. To analyze antifungal susceptibility and evaluate the associated risk factors in Candida spp.strains isolated from vaginal secretions in women aged 22 to 65 years and to develop an algorithm for the identification and characterization of Candida spp. strains. The methodology has a descriptive, prospective, cross-sectional approach, involves the collection of a vaginal swab sample, the information and data were obtained during the course of the research. The results obtained represent the susceptibility profile circulating in this health center and could be potentially useful for monitoring and guiding treatment. When testing for susceptibility to antifungals C. albicans, C. parapsilosis were sensitive to fluconazole and voriconazole in 100%, C. glabrata was SDD for fluconazole, voriconazole has no cut-off point and C. krusei presented sensitivity to voriconazole, fluconazole shows intrinsic resistance. krusei presented sensitivity to voriconazole, fluconazole shows intrinsic resistance, within the risk factors we have the active sexual life, diabetes, which proposes to implement preventive measures to avoid recurrence of vaginal infections, for this reason it is necessary to expand the research in other populations such as pregnant women, patients with Human Immunodeficiency Virus, and minors.
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    EVALUACIÓN DE LA CONFIABILIDAD DE LA METODOLOGÍA MANUAL FRENTE A LA METODOLOGÍA AUTOMATIZADA EN LA IDENTIFICACIÓN DE CÁNDIDA ALBICANS
    (Universidad Técnica de Ambato / Facultad de Ciencias de Salud / Centro de Posgrados, 2024-10-01) Torres Valdez, Lissette Estefanía; Guangasig Toapanta, Víctor Hernán
    Candida albicans is the main species related to most superficial and deep infections caused by yeasts, therefore it is necessary to have tests that are fast and accurate for timely microbiological identification through the reliability of both manual methodologies and automated methodologies such as the VITEK system and thus establish which is the best option to use in the laboratory, THE objective was to evaluate the reliability of manual methodology versus automated methodology in the identification of Candida albicans. The methodology used was a cross-sectional observational analytical study, with a quantitative approach since data collection was used based on numerical measurement and statistical analysis, it is non-experimental, according to the chronology the research is prospective, the inclusion and exclusion criteria were analyzed. The results were as follows: from 100 microbiological samples of fungi where 82% (n=82) corresponds to isolates of the albicans species, 18% corresponds to other species, which are: tropicalis (n=10), glabrata (n=5) and parapsilosis (n=3), was isolated in 82 samples of the microorganism to be identified of which it is observed that 73. 17% were isolated from vaginal secretion samples, 10.98% from skin secretions, 8.54% from seminal secretions, and 3.66% from oral and intestinal secretions. Of the total of 82 positive samples for Candida albicans, the conventional method (CHROMagar) and the automated method (VITEK) gave 70 correct identifications of the yeast and one incorrect identification of the yeast. On the other hand, CHROMagar identified 3 positive results that VITEK did not and VITEK identified 8 positive results that CHROMagar failed, the Kappa statistic (κ),. κ =0.093 indicates that there is a slight concordance between the CHROMagar and VITEK methods. The results indicate that there is no statistically significant agreement between the CHROMagar and VITEK methods for identifying Candida albicans. The odds ratios suggest some level of association, based on the Kappa statistic, an acceptable to moderate agreement between the two methods is proposed..