Ciencias de la Salud
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Item “Determinación de Salmonella Typhi y su relación a enfermedades diarreicas en los comerciantes del mercado mayorista de la cuidad de Ambato”(Universidad Técnica de Ambato - Facultad de Ciencias de la Salud - Carrera de Laboratorio Clínico, 2019-05-01) Coba Mejía, Nelson Xavier; Guangasig Toapanta, Víctor HernánBqf. Mg.Gastrointestinal diseases are one of the main public health problems in Ecuador. They are transmitted, either by fecal-oral route, or by the consumption of contaminated water or food. Its incidence as its prevalence depends on the socioeconomic level of the patients and mainly affects the child population. The pathogens involved are viruses, parasites and bacteria. The identification of these, in clinical laboratories, focuses on clinical pathogens such as: Salmonella, Shigella, Escherichia, Vibrio, Campylobacter and Yersinia. The objective of the present study was to identify S. Typhi, as a causative agent of gastrointestinal infections in wholesale market traders, by means of the Rapid S. Typhi Antigen Test Card immunochromatography test, to verify by biochemical tests the specificity of the test and to establish the profiles of sensitivity and / or resistance presented by the bacterium isolated by antibiogram by the Kirby-Bauer method. Fifty patients were surveyed and the test was performed with Rapid S. Typhi Antigen Test Card immunochromatography in search of S. Typhi. 20% of the samples tested positive for S. typhi. The biochemical tests performed in the media TSI, SIM, Urea, Citrate, Malonate, RM-VP gave 100% positivity for S. Typhi. The antibiogram reflex resistance of 20% of the strains to Ampicillin and 100% sensitivity to Ceftazidime, Amoxicillin + Ac. Clavulanic, Trimethoprim / sulfamethoxazole, Tetracycline, Gentamycin, Ac. Nalidixico and Ceftriaxone. It was established that of the 50 cases of patients with diarrheic processes, only 20% was due to S. Typhi bacteria.Item Determinación de Enterobacterias Mediante Coprocultivo y su Relación con Gastroenteritis no Parasitaria en Pacientes Adultos que Residen en el Cantón Pujilí - Cotopaxi(Universidad Técnica de Ambato-Facultad de Ciencias de la Salud-Carrera Laboratorio Clínico, 2016-11-01) Herrera Durán, Magaly Johana; Salazar Garcés, Dolores Krupskaya Lcda. Mg.The research project was conducted in the area of Microbiology play an important role when analyzing biological samples, which helps with the diagnosis of the origin of infectious diseases. The objective of this project was to investigate the types of bacteria that are causing acute gastroenteritis bacterial disease (GEBA), it was done with stool samples collected from residents adult patients from Pujili Canton by a pure culture, on selective and specific media, with an incubation of 24 to 48 hours in which to get a better result was complemented by biochemical tests as TSI (triple sugar iron), SIM (sulfur, indo, mobility), urea and citrate, of this way to identify the gender and species of the bacteria and finally a reseeding was done in the Agar Mueller Hinton to verify the susceptibility testing with the use of sensitivity discs broad spectrum, for which a bacterial dilution was performed with the standard 0.5 of the MacFarland turbidity scale. The result was a population of 450 patients between 20 and 65 years old who attend to the Clinical Laboratory, after inclusion and exclusion criteria, a sample of 80 patients women / men who have had symptoms of GEBA was obtained. So in this research project it was concluded that, 44 samples correspond to 55% which had growth of Escherichia. coli, 24 samples are equivalent to 30% of Salmonella spp development, and finally 12 samples corresponding to 15% of growth of Klebsiella oxytoca, reaching 100% of total samples, investigating the predisposing factors for appearance of GEBA, it was concluded that most cases are due to food contamination , and lack of personal hygiene