Evaluación del efecto del jugo de tuna (Opuntia ficus-indica l. mill) y vitamina E sobre los parámetros de calidad seminal en la crio preservación de semen ovino

Abstract

The present investigation was carried out with the objective of evaluating the effect of prickly pear juice (Opuntia ficus-indica L. Mill) and vitamin E on semen quality parameters in cryopreservation of ovine semen. For which three treatments were applied: Tc: the control treatment where a commercial diluent (Triladyl) was obtained, Tt: Tuna juice was obtained as a natural diluent and Tt+ VE: the diluent based on tuna juice was applied. and vitamin E was added. We worked with 3 rams and 1 female, semen was extracted three times a week and a pool was made with the three ejaculations, therefore we worked with heterospermic semen. A study with a random design was applied with a 3*3 factorial arrangement with a time factor at 0 - 2 -4 hours post-thawing. For the results in the comparison of means between the treatments, it was analyzed by means of an analysis of variance and contrasts with the Tukey test in the Infostat program, the differences were considered statistically significant at the level of P < 0.05. Semen was analyzed in two stages with corresponding variables, fresh semen volume, translucency, color was evaluated macroscopically and concentration, mobility, morphology, vitality was analyzed microscopically and in post-thawed semen at 0-2-4 hours concentration, mobility, morphology and vitality. Concluding that Tuna juice and vitamin E do influence seminal quality parameters.