Efecto de la aplicación de gnrh sintética sobre la calidad seminal y caracteristicas reproductivas de carneros alimentados con alfalfa contaminada con Pseudopeziza medicaginis

Abstract

This work was carried in Technical University of Ambato where nine (3 for each treatment) adult rams mest with a live weight of approximately 40kg and 2 years old were used; the animals went through a process of adaptation and training with a duration of 20 days for it: This objective of this research was to evaluate the effect of the application of synthetic GnRH (buserelin acetate) on the seminal quality and reproductive characteristics of rams fed with alfalfa contaminated to 70% of Pseudopeziza medicaginis. The project was carried out in a completely randomized design. The frequency of GnRH administration and seminal extraction (T0: Control, T1: Alternate, T2: Continuous) by artificial vaginas for the evaluation of: volume, mortality, concentration, morphology: loose head of sperm (MCS), small tail (CP), beheaded (DC), crooked tail (CT), bent tail (DAG defect) (CDD), tail rolled up (CE), mass mobility, individual mobility on the other hand was also evaluated: scrotal circumference (EC), testicular volume (VT) and copulatory capacity (Ex. Female, N° jumps, ejaculate, time) of which the following results were obtained: with respect to sperm morphology referring to a loose head of sperm (indicator 4), it showed a significant difference (P=0,0048) between treatments finding lower percentages of loose heads of sperm in animals that were administered a dose (12μg) of half-hour buserelin acetate before extraction for 7 days in a row (T2); as soon as regarding mass and individual mobility, differences were observed (P=<0,0001), with greater mobility in T1 (4) compared to T0 and T2. It is concluded that the administration of buserelin acetate at a dose of 12μg /ram IM acted efficiently decreasing the index of loose heads when administrated 30 minutes before seminal extraction for 7 continuous days (T2) and as a consequence in both mass and individual mobility. What is feasible in rams fed alfalfa (Medicago sativa) contaminated with 70% of the fungus Pseudopeziza medicaginis