Efecto de glucosinolatos sobre la inhibición del crecimiento micelial de Phytophthora infestans de Bary in vitro.

Abstract

This research was conducted in the laboratories of Plant Health and the bio-inputs laboratory at the Technical University of Ambato, Faculty of Agricultural Sciences, located in the Cevallos Canton on the Quero road at a distance of 20 km from the city of Ambato, at an altitude of 2850 meters above sea level. The objective was to evaluate the effect of glucosinolates on the inhibition of the mycelial growth of Phytophthora infestans De Bary in vitro. Two species were used in this research: Broccoli (Brassica oleracea var. Italica) and Brussels sprouts (Brassica oleracea var. Gemmifera) at concentrations of 10%, 20%, and 30% of each glucosinolate extract. Data were collected over 9 days, equivalent to 216 hours, at 24-hour intervals. To measure the mycelial growth, two lines were drawn in the Petri dish passing through the center of the microorganism's growth halo. Statistical analysis was performed using a completely randomized design (CRD) with 6 treatments and one control, with three replications. The statistical package INFOSTAT was used, and Tukey's significance tests at 5% were applied to variables showing statistical differences. The treatment E2C3, corresponding to Brussels sprouts glucosinolate at 30%, reported better results for the mycelial growth and inhibition percentage with averages of 12.33 mm and 81.19%, respectively. In contrast, the control showed a mycelial growth of 65.55 mm and an inhibition percentage of 0%. According to the results obtained in the research, the use of Brussels sprouts glucosinolates is an alternative for the biological control of P. infestans. KEYWORDS: Mycelial growth, glucosinolates, inhibition, percentage