Ciencias Agropecuarias
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Item Caracterización molecular de genes de avirulencia del agente causal del moho gris de la hoja de Solanum lycopersicum L en la provincia de Tungurahua(2022-08) Flores Yanchaliquin, Diana Carolina; León Gordón, Olguer AlfredoCladosporium fulvum or leaf mold is a pathogen described by Cooke in 1883 that develops easily in tomato crops due to favorable temperature and humidity conditions. For the present investigation, the objective was to "Molecularly characterize avirulence genes of the causal agent of the mold of the leaves of Solanumlycopersicum L in the province of Tungurahua". For the molecular characterization of genes, fungal DNA was extracted from each canton (Ambato, Baños, Cevallos, Mocha, Patate, Pelileo, Píllaro, Tisaleo), which consisted of lyophilizing the mycelium, crushing and placing the respective buffers (Buffer A Naoh + Tween 20 and Buffer B Tris Hcl + Edta) and the extracted DNA was stored at -20°C. For amplification using conventional PCR, the amplification conditions for each Avr andEcp and their process were standardized, using dNTPs, Taq DNA Polymerase,forwrad-reverse primers, and ultrapure water with 25 μl of reaction. For visualization,1% agarose gels were made with the running buffer TAE 1X and a 100bp molecular marker (Invitogen) was used. Having as results the presence of the Avr2 and Avr4E avirulence gene, thus also verifying the absence of Avr4 and Avr9, while for extracellular proteins Ecp2, Ecp4 and Ecp5 are presented so that for Ecp1 it does notrecognize the presence of the gene