Ciencias Agropecuarias

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Author: search.filters.author.Garcés Arias, Xiomara SthefannySubject: search.filters.subject.ESCHERICHIA COLI

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    Determinación de la presencia de genes de resistencia a betalactámicos y evaluación de diversidad clonal en aislados de Escherichia coli de origen canino de la ciudad de Ambato
    (2020-03-19) Garcés Arias, Xiomara Sthefanny; Calero Cáceres, William Ricardo
    The evolution and spread of multiresistant bacteria is considered prioritary, considering their impact in public health and animal safety worldwide. Therefore, the detection of reservoirs, routes of dissemination and evolution of these bacteria is aimed in order to limit their negative effects on public and animal health. Extended-spectrum-betalactamase Escherichia coli producer is considered an emerging health problem. In Ecuador, there are few available data on the molecular epidemiology of those of ESBL- E. coli producing bacteria in animals and environment. In the present study, the presence of beta-lactam resistance genes was evaluated as well as the clonal diversity of E. coli isolates of canine origin from the city of Ambato. The highest presence of beta-lactam resistance genes occurred in dogs with owners, in contrast to stray dogs. The most frequently detected genes were blaCTX-M and blaTEM. The blaSHV and blaCMY genes had a lower detection rate, however, these were found in combination with the blaCTX-M and blaTEM genes, indicating that they are isolates with considerable virulence. The analysis of clonal relatedness through the amplification of palindromic repetitive regions (GTG5) detected that the isolates comes from different lineages, discarding the hypothesis that suggest the presence of multi-resistant clones with a common ancestor, suggesting that their genotypes were acquired through recent horizontal genetic transmission. Dogs can act as a silent reservoir of microorganisms with antibiotic multiresistance genotypes. It is necessary an extended review of waste management procedures is necessary, as well as interactions with humans and animals under certain circumstances like immunological disorders.