Ciencias Agropecuarias
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Item Identificación de parásitos gastrointestinales en venados de cola blanca (Odocoileus virginianus) por diferentes métodos coprológicos en el Zoocriadero La Casa del Venado -Cayambe(2023-03) Herrera López, Amanda Carolina; Quinteros Freire, Christian AndrésThe objective of this research was to identify gastrointestinal parasites in white-tailed deer (Odocoileus virginianus) by different coprological methods. Thirty-eight deer samples were processed, 16 of the samples belonged to the adult category, 16 to the juvenile category and 6 to the calf category. The samples were evaluated by two coprological flotation methods: Sheather flotation based on a sucrose solution at a density of 1.20 and Faust flotation based on a solution with Zinc sulfate at a density of 1.18. The data obtained were compiled in Excel to make frequency tables and determine if there are differences between the 2 coprological methods for the observation of parasites. The parasites that occurred most frequently were the nematodes Strongyloides spp, Capillaria spp, Trichostrongylus spp, Ostertagia spp, Oesophagostomum spp and the protozoan Eimeria spp. Strongyloides spp. was the parasite that presented the highest percentage in the 3 categories of adults (79% in Sheather and 83% in Faust), hatchlings (72% in Sheather and 83% in Faust), and juveniles (92% in Sheather and 94% in Faust). Nine student t-tests were performed for Strongyloides spp, Trichostrongylus spp and Eimeria spp parasites, which showed that there are differences in the methods, with this and the counting of eggs in the McMaster chamber, it was evidenced that the most effective method is the Faust method due to its capacity to provide samples with less sediment and preserve each parasite structure. All parasites found were classified by groups and described morphologicallyItem Identificación de parásitos con diferentes métodos coprológicos en muestras de reptiles en el Vivarium de Quito(2021-08) Núñez Alverca, Karla Alejandra; Almeida Secaira, Roberto IsmaelThe current study has the objective to identify gastrointestinal parasites through three coprological methods in reptile fece samples of Vivarium Quito. 118 reptile samples were processed and analyzed; which 81 belong to 17 suborder Ofidia species, 23 samples belong to 7 different order Chelonia species and 14 samples belong to 5 suborder Saurus species. From this sample analysis, 88 (74.6%) samples result positive to any kind of parasite. For sampling, physician restraint were made according to the kind of reptil; it was used hooks or herpetological tubes to manipulate snakes, in case of poisonous specimens, they were managed by authorized staff, turtles and lizards were contained through manual restraint, turtles from the base of the tail or body and lizards avoiding his tail restraint, holding its paws. The samples were recolected with tongue depressors, kept in ziploc bags and kept refrigerated, then they were tagged with the scientific name of the animal, number of alive, area where are located in Vivarium and date of collection, transportation was made through of a cooler with cooling gels at a temperature of 4°C. The direct coprological, float and sedimentation methods were applied in each sample taken. As a result, 40 different kind of parasites were identified, 6 genera of Protozoan, 13 of Nematodes, 12 of Trematodes, 5 of Cestodes, 2 of Acantocephalus, 1 of Mites and 1 of Pentastomide type. From these results, the most frequently parasites were Blastocystis spp Protozoan (25%), nematodes within order of pinworms (23.9% egg) (11.4% adult), Balantidium sp (11.4%), Metamonádidos (10.2%), Strongyloides (9.1%), Kalicephalus sp (8%), Nyctotherus spp, Entamoeba sp., Ophionyssus natricis y Rhabdias spp these four kind of parasites with 6.8%. All identified genres were characterized morphologically according to their classification, presentation or status, shape, colour, size, and the coprological method which were identified. Finally, it was resulted that order Chelonia (82.6%) have the most frequency of parasites, then Squamata (72.6%), but separately Saurus has 100% of parasitism and Ofidia species 67.9%. In conclusion, there is a great variety of gastrointestinal parasites in reptiles of the study and it is priority to have a preventive deworming plan for avoiding being prone to future pathologies in these animals.Item Identificación de parásitos con diferentes métodos coprológicos en muestras de reptiles en el Vivarium de Quito(2021-06) Núñez Alverca, Karla Alejandra; Almeida Secaira, Roberto IsmaelThe current study has the objective to identify gastrointestinal parasites through three coprological methods in reptile fece samples of Vivarium Quito. 118 reptile samples were processed and analyzed; which 81 belong to 17 suborder Ofidia species, 23 samples belong to 7 different order Chelonia species and 14 samples belong to 5 suborder Saurus species. From this sample analysis, 88 (74.6%) samples result positive to any kind of parasite. For sampling, physician restraint were made according to the kind of reptil; it was used hooks or herpetological tubes to manipulate snakes, in case of poisonous specimens, they were managed by authorized staff, turtles and lizards were contained through manual restraint, turtles from the base of the tail or body and lizards avoiding his tail restraint, holding its paws. The samples were recolected with tongue depressors, kept in ziploc bags and kept refrigerated, then they were tagged with the scientific name of the animal, number of alive, area where are located in Vivarium and date of collection, transportation was made through of a cooler with cooling gels at a temperature of 4°C. The direct coprological, float and sedimentation methods were applied in each sample taken. As a result, 40 different kind of parasites were identified, 6 genera of Protozoan, 13 of Nematodes, 12 of Trematodes, 5 of Cestodes, 2 of Acantocephalus, 1 of Mites and 1 of Pentastomide type. From these results, the most frequently parasites were Blastocystis spp Protozoan (25%), nematodes within order of pinworms (23.9% egg) (11.4% adult), Balantidium sp (11.4%), Metamonádidos (10.2%), Strongyloides (9.1%), Kalicephalus sp (8%), Nyctotherus spp, Entamoeba sp., Ophionyssus natricis y Rhabdias spp these four kind of parasites with 6.8%. All identified genres were characterized morphologically according to their classification, presentation or status, shape, colour, size, and the coprological method which were identified. Finally, it was resulted that order Chelonia (82.6%) have the most frequency of parasites, then Squamata (72.6%), but separately Saurus has 100% of parasitism and Ofidia species 67.9%. In conclusion, there is a great variety of gastrointestinal parasites in reptiles of the study and it is priority to have a preventive deworming plan for avoiding being prone to future pathologies in these animals.