Ciencias Agropecuarias
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Item Impacto ambiental de la inclusión de saponinas en dietas fibrosas sobre la función ruminal y mitigación de emisiones de dióxido de carbono y metano entérico en bovinos(2020-10) Veloz Vargas, César Xavier; Barros Rodríguez, Marcos AntonioThe objective of the present investigation was to evaluate in vitro the effect of the inclusion of saponins and GISAG in fibrous diets on ruminal function, mitigation of GHG emissions, as well as ammoniacal nitrogen production; 6 bulls with ruminal cannulas were used, fed with a base diet of 50% maralfalfa grass (Pennisetum purpureum) plus 50% corn bale (Zea mays), the treatments were: T control only base diet, T2 base diet plus 3 % of saponins, T3 base diet of 3% GISAG (Saccharomyces cerevisiae) and T4 base diet plus 3% saponins and 3% GISAG, a completely randomized design of 4 treatments and 6 repetitions was handled, the variables were analyzed through an analysis of variance in the SAS PROC GLM program and for the comparison of means, the 5% Tukey test was performed, the animals were housed in individual pens and water at will, with respect to the variable in vitro digestibility of MS showed no differences between treatments (P = 0.7684) while the population of protozoa in the rumen evaluated at different hours of fermentation, showed no differences (P> 0.05), except for Entodiniomorphic protozoa at hour 24 (P = 0.0001), the decrease in vitro of this population of protozoa observed at 24 hours in T2, with respect to gas production, was less (P = 0.0111) in the T2 treatment with 37.2 mL of gas / 0.5g DM fermentable, the methane production did not show differences between the evaluated treatments (P = 0.3592), however, the CO2 production was lower (P = 0.0196 ) in T2, the volatile fatty acids did not observe differences between the treatments; however, in ammoniacal nitrogen it was higher (P> 0.05) in treatments T2, T3 and T4, with T2 being the one with the best behavior in GHG mitigation and nitrogen utilization for the productive benefit of the animal.Item Influencia de enzimas exógenas O Saccharomyces cervisiae en dietas fibrosas sobre la fermentación ruminal y producción de gas in vitro en ovinos(2018) Razo Barrera, Jessica Yesenia; Barros Rodríguez, Marcos A.The purpose of this investigation was to evaluate the effect of the exogenous enzymes or Saccharomyces cerevisiae in fibrous forages over ruminal fermentation in ovines. The experiment was carry out in the Faculty of Agricultural Sciences of the UTA. Two experiments were carried out, 1) 4 mixed-breed male animals with an average live weight of 30kg housed in metabolic cages and 2) 4 mixed-breed male ovines provided with a fistula with a cannula in the rumen (2 inches of internal diameter and 5 inches of external diameter). The animals were distribute randomly in a 4x4 Latin square design. The ovines were fed an integral diet based on barley stubble. Four treatments were evaluated; T1: control diet (without enzymes or yeasts), T2: diet with enzymes (2 mg / kg), T3: diet with yeasts (1.5 mg / kg) and T4: diet with enzymes and yeasts (2 mg / kg enzymes + 1.5 mg / kg yeast). Voluntary intake, pH, ammoniacal nitrogen production, VFAs, kinetics of ruminal degradation in situ and in vitro, gas production were determined. Voluntary nutrient intake showed no differences between treatments (P> 0.05). Voluntary consumption of digestible nutrients was higher for T1, T2 and T4. Digestibility was higher for T1, T2 and T4 in MS and MO, while for NDF and FDA it did not show differences between treatments P = (0.1035 and 0.2029). The in situ ruminal degradation of the MS was greater for T2, T3 and T4. Regarding in vitro gas and methane production, the lowest production observed was in T1, T2 and T4. Finally, the pH remained at an average of 7.3 - 7.6, which is favorable for enzymes and optimal for ruminal microorganisms; the ammonia nitrogen increased in the T2 and the production of VFAs did not show differences between treatments (P> 0.05). Under the conditions of this study it was concluded that the addition of exogenous enzymes to the diet favored food consumption, fiber digestibility, degradation and pH, thus creating an optimal environment for the development of ruminal microorganisms and therefore a better synthesis of the protein; resulting in a decrease in gas production at the rumen level.