Caracterización de extractos obtenidos a partir de Tarqui Myrsine andina (Mez) Pipoly, planta nativa del cerro Teligote

Abstract

The objective of this research work was to characterize the extracts obtained from TARQUI Myrsine andina (Mez) Pipoly, a native plant of Cerro Teligote, for the optimization of the extraction process, three factors were considered: solvent (ethanol, methanol, at a concentration of: 25, 50 and 75 percent and pure) and water; temperature (30, 40, 50, and 60 degrees centigrade) and time (5.10 and 20 min), the extraction method was standardized for polyphenons, 50 percent ethanol, 50 degrees centigrade and 5 min, while for flavonoids it was 75 percent ethanol, 50 degrees centigrade and 20min. When comparing the different solvents for the extraction of polyphenols, the highest percentage of plant wear was obtained which was 98.91 percent for flavonoids and 97.74 percent for polyphenols and thus obtain the most concentrated extract with the minimum degradation of its compounds during the production process. The concentration of polyphenols in the tarqui extracts was determined by the Folin Ciocalteu method, and the ultraviolet-visible spectrophotometry method was used for the concentration of total flavonoids. The separation and identification of the polyphenols and flavonoids present in the extract, high performance liquid chromatography (HPLC) with two standards was used.