Tesis Medicina Veterinaria

Permanent URI for this collectionhttp://repositorio.uta.edu.ec/handle/123456789/36415

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Efecto de adición de agua de coco centrifugado en diluyente comercial como potenciador de la calidad seminal en caninos post refrigerado
(2024-08) Romero Rosero, Luis Fernando; Aragadvay Yungan, Ramon Gonzalo
The objective of this research was to evaluate the effect of adding 5% and 10% centrifuged coconut water (CCA) to a commercial diluent as an enhancer of postchilled canine seminal quality. Three treatments were evaluated: T0 (semen+diluter), T1 (semen+diluent+5%ACC) and T2 (semen+diluent+10%ACC), the variables measured were macroscopic (volume, color/opacity, pH) in fresh semen, microscopic in post-chilled semen (concentration, mobility mass, individual mobility, vitality and morphology) and complementary tests: Host Test and Acrosome Integrity Test and were analyzed in three times 24, 72 and 120 hours, the extender was mixed with the semen in a proportion of 2:1, The coconut water was previously filtered through Whatman No. 4 filter paper and centrifuged and added to the mixture of the diluent and ejaculated semen obtained from the cross-breed canine under study. The results were as follows: average volume of ejaculated semen was 5.83 mL, whitish color, and pH of 6.00, sperm concentration (millions of sperm/mL) between 227.00 x106- 288.83 x106, there was a significant difference in T1 at 24 hours in the variables mass mobility, individual mobility, vitality, morphology, Host Test , Acrosome integrity test with values of 66.67%, 78.33%, 82.50% and 83.33%, 70.00%, 42.50% respectively, which means that they showed the best percentages in each of these variables. Concluding that the addition of 5% of centrifuged coconut water to the extender and ejaculated semen maintains refrigerated canine semen quality up to 24 hours postcooling, unlike the variable integrity of the acrosome, which is drastically affected by the lack of use of cryoprotectants to the experiment.
Efecto del extracto de Maca (Lepidium meyenii) sobre los niveles de testosterona y parámetros de calidad seminal en conejos (Oryctolagus Cuniculus) mestizos adultos
(2024-08) Lema Cerón Cynthia Giuliana, Cynthia Giuliana; Aragadvay Yungan, Ramon Gonzalo
Maca (Lepidium meyenii) is a hypocotyl native to the Andean region that has been cultivated for 2000 years. Maca is a root rich in essential amino acids, fatty acids and other nutrients. In addition, it contains abundant bioactive compounds responsible for various benefits for fertility and as an aphrodisiac product. Therefore, the objective of our research is to find out the effect of Maca on semen quality parameters and testosterone levels. For this reason, 4 treatments have been applied: T0: control group, T1: 0.1% Maca extract, T2: 0.2% Maca extract and T3: 0.3% Maca extract. It was administered to 48 6–7-month-old male rabbits from a cross between New Zealand and Belier. Through a complete randomized block design with 4 blocks per treatment consisting of 3 animals per block, the data were evaluated with Tukey's statistical test (p<0.05) with the InfoStat statistical program. Testosterone evaluation was carried out in two doses each for 15 days, taking a sample for each block of each treatment, which were evaluated in the laboratory with an Electrochemiluminescence technique, while semen samples were taken every day. Good results were obtained with the T2 and T3 treatments with the inclusion of 0.2% - 0.3% in the semen quality parameters, while in the testosterone tests the effect of Maca on this variable was not seen.
Evaluación de los cambios en la calidad seminal de conejos de la raza neozelandés (Oryctolagus cuniculus) de 6 meses, 1 año y 2 años de edad
(2024-08) Molina Chicaiza, Daniela Mishell; Aragadvay Yungan, Ramon Gonzalo
The present investigation focuses on the evaluation of changes in semen quality in New Zealand breed rabbits (Oryctolagus cuniculus) of 6 months, 1 year and 2 years of age. The main objective of the study is to analyze how age affects semen quality parameters in this specific breed. For this purpose, weekly fresh semen collections were performed using an artificial vagina and a female dummy, evaluating both macroscopic and microscopic semen parameters. Analyses included volume, color, motility, sperm consistency, sperm morphology and appearance of fresh semen. The results showed that 1-year-old rabbits presented better semen quality in terms of vitality and concentration, with a lower amount of sperm abnormalities compared to 6-month-old and 2-year-old rabbits. It was determined that age is a significant factor in semen quality in New Zealand breed rabbits. In addition, it is emphasized that dietary supplementation and collection technique do not significantly alter the parameters evaluated, as long as adequate conditions are maintained during the process.
Evaluación del efecto de la semilla de linaza (Linum usitatissimum) sobre los niveles de testosterona y parámetros productivos en conejos mestizos (Oryctolagus cuniculus) durante la fase de engorde
(2024-08) Sánchez Díaz, Leonela Mileidy; Aragadvay Yungan, Ramon Gonzalo
This study investigated the effect of including flaxseed (Linum usitatissimum) in diets of rabbits during the fattening phase, evaluating testosterone levels and productive parameters. Four treatments were used: T0 as control, and T1, T2, and T3 with additions of flaxseed at 3%, 6%, and 9%, respectively, all supplemented with alfalfa. Treatments (T0, T1, T2, and T3) were assigned using a randomized block design. The research was conducted over 30 days. Significant differences were observed, such as in the final weight of the rabbits, which was higher in T2 (2235.11 g) and T3 (2239.89 g) compared to T0 (1822.11 g) and T1 (2030.56 g). Food consumption varied significantly between treatments, with higher values in T2 (2187.88 g) and T3 (2282.68 g). Daily weight gain was highest in T3 (33.42 g/day), followed by T2, suggesting a potential positive effect of flaxseed on feed efficiency. There were no significant differences in feed conversion (P = 0.2583), indicating that efficiency was not affected by flaxseed addition. Mortality was low in all groups (1.23% in T0 and T1; no mortality in T2 and T3). Testosterone levels showed numerical increases with flaxseed supplementation, though without significant statistical differences. The study also evaluated carcass quality and internal organs, highlighting potential beneficial effects of flaxseed on basal metabolism and overall rabbit health.