Carrera Ingeniería Bioquímica

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    Evaluación de la actividad antibacteriana y antiinflamatoria in vitro del extracto vegetal seco de mangle rojo (Rhizophora mangle L.)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2018-07) Acosta Sánchez, Gissela Maribel; López Hernández, Orestes Darío
    In the present study, the antimicrobial and anti-inflammatory activities of the dry total extract of red mangrove (Rhizophora mangle L), whose tests were carried out in vitro, were evaluated. The biological activities tested are possibly due to the polyphenols contained in this plant extract. The polyphenolic quantification of the powder extract was carried out by the Folin-Ciocalteu method giving a concentration of 338,31 more less 4,24 mgGAE per g. The antimicrobial activity was performed by disc-plate diffusion method, 5 pathogenic microorganisms were used, the results obtained indicated that the plant extract inhibits the microbial growth of Gram-positive bacteria, among them Listeria monocytogenes, Bacillus cereus and Staphylococcus aureus, while that for Gram-negative bacteria a positive test for inhibition of bacterial growth was not evident, among them Escherichia coli and Pseudomonas aeruginosa, as a positive control, Gentamicin was used at 500 μg per ml. For Gram-positive bacteria, the minimum inhibitory concentration (MIC) whose results were 11,23 mg per ml; 6,92 mg per ml and 5,63 mg per ml S. aureus, L. monocytogenes and B. cereus, respectively. The anti-inflammatory activity was evaluated on human erythrocytes, by means of the membrane stabilization method, where the percentage of the anti-inflammatory capacity was higher than the positive control (aspirin) in the concentrations of 1; 1.5 and 2 mg per ml whose results were 68,51 percent; 83,34 percent and 84,67 percent respectively, while the positive control gave a percentage of anti-inflammatory activity at the same concentrations of 54,75 percent; 64,75 percent and 74,49 percent.