Carrera Ingeniería Bioquímica

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Author: search.filters.author.Espinosa Panchi, Melany NoemíSubject: search.filters.subject.EXTRACTOS VEGETALES

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    Extracción, cuantificación y caracterización de compuestos fenólicos extraídos a partir de la Arquitecta (Lasiocephalus ovatus Schltdl), planta nativa del cerro Teligote
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2020-01) Espinosa Panchi, Melany Noemí; Morales Acosta, Dayana Cristina
    L. ovatus is a native plant from Teligote’s hill (1°22'39.2"S; 78°33'54.7"O; 3633 m.s.n.m.). It has used by locals people as diuretic and depurative. The aim of this research study was extract, quantify and characterize phenolic compounds obtained from L. ovatus. Dried and crushed leaves were used from the first step of optimization processed that consist of choosing the major solvent (water, methanol and ethanol), time (5, 10, 20 min) and temperature (30, 40, 50, 60 Celsius degrees). The best conditions to extract polyphenols in L. ovatus matrix were ethanol 50 percent (v/v), 50 Celsius degrees and 20 min, while by flavonoids were ethanol 50 percent (v/v), 60 Celsius degrees and 5 min. Depletion of vegetal material was made with 4 and 5 successive extractions for polyphenols and flavonoids, respectively. TLC and HPLC techniques were used to characterize phenolic compounds in the extracts of L. ovatus. Furthermore, anti-inflammatory activity was tested in vitro by HRBC obtained of were 98,04 more less 0,44 for flavonoids and 97,29 more less 0,12 for polyphenols and protein denaturation method with values of 36,30 more less 0,29 for polyphenols and 28,71 more less 0,63 for flavonoids. Antioxidant activity showed values 86,46 more less 0,83 y 84,18 more less 0,57 for polyphenols and flavonoids respectively. In this way it was concluded that L. ovatus presents in its structure phenolic components that confer important biological activities (anti-inflammatory and antioxidant activity) thus supporting its use inside and outside the community of Teligote as a possible natural alternative.