Carrera Ingeniería Bioquímica

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Author: search.filters.author.Moreta Pacha, Erika JazmínSubject: search.filters.subject.CAPACIDAD ANTIOXIDANTE

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    Capacidad antioxidante in vitro de Valeriana microphylla y Valeriana plantaginea en células cancerígenas
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2021-01) Moreta Pacha, Erika Jazmín; Vargas López, José Homero; Carrero Castillo, Yenndy Naygith
    Free radicals play an important role in cell metabolism, proving to be beneficial or deadly for the body. Due to their aggressive nature, these can contribute to the genesis of cancer; however, there are defense strategies using antioxidants. Plants play an important role within therapeutic resources, which are bioactive agents used in pharmaceutical laboratories in research and therapeutic proposals for various pathologies such as breast cancer. The objective of the research was based on determining the antioxidant capacity of Valeriana microphylla and Valeriana plantaginea extracts in MFC-7 (breast cancer) cells. The extracts were made in two groups, the first was the combination of Leaves Stems and the second roots for each species; For the determination of total solids, water was used as a solvent and for cell culture ethanol, with the following results: the total soluble solids with the best performance were obtained in Valeriana plantaginea Leaves Stems. The cytotoxic activity was quantified by the MTT method; where the extract of Leaves Stems of Valeriana plantaginea, reached the highest percentage. Through immunohistochemical staining, the expression of ROS, MITOSOX, GLUTTATION was evidenced using specific monoclonal antibodies for each of the proteins, which show significant differences compared to the control. The antioxidant capacity was determined using the Trolox technique, showing a low antioxidant capacity for both species of valerians. The results allowed us to infer the therapeutic potential of these valerian species, however, complementary studies should be carried out.