Carrera Ingeniería Bioquímica

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Capacidad proapoptótica in vitro de Valeriana rígida y Valeriana decussata en células cancerígenas
(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2021-01) Williams Ibarra, Jeniffer Andrea; Carrero Castillo, Yenddy Nayghit
Cancer represents a public health problem level world, with high incidence and mortality rates in developed and undeveloped countries. Currently there are different therapeutic treatments to eliminate or prevent the proliferation of cancer cells, however, the most are invasive, and they have side effects. Today there are natural therapeutic alternatives with the aim of develop more efficient and less invasive treatments to fight cancer. Faced with this problem, the research done it is based on in vitro evaluation of the proapoptotic and cytotoxic capacity of valerian extracts on the breast cancer cell line. Cell viability assays (MTT), immunohistochemistry assays were performed by fluorescence microscopy and quantitative reverse transcription PCR for gene expression of proteins. The results obtained showed the decrease in cell proliferation and variability in the gene expression of apoptotic proteins in breast cancer cells after being treated with optimal doses of Valeriana rigid and Valeriana decussata extracts.