Carrera Ingeniería Bioquímica
Permanent URI for this collectionhttp://repositorio.uta.edu.ec/handle/123456789/809
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Item Evaluación de la capacidad de remoción de cadmio mediante levaduras (Metschnikowia pulcherrima, Lecythophora ps.LE034), aisladas del volcán Tungurahua.(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2016-07) Aguas Bonilla, Daniela Magaly; Dugarte Jiménez, Nahir YerelyThis study determined the removal capacity of cadmium through the use of biomass of yeasts isolated from the Tungurahua volcano. It was chosed two species Metschnikowia pulcherrima, Lecythophora sp. LE034 and a third commercial species as reference Saccharomyces cerevisiae. The determination of cadmium in the removal process was tested by the modification of the colorimetric method of the dithizone by Ecuadorian technical standard INEN 0982. The number of colony forming units (cfu) and growth factor of a series of dilutions were determinated through McFarland curve and count per diffusion in plate. The percentage of inhibition of growth against cadmium using concentrations from 107 up to 1,07 µg/l, was between 85% and 95%, being the yeast Lecythophora sp. LE034 the most susceptible to inhibition. The removal process showed that the three yeasts are able to efficiently remove between 88-89% of cadmium.Item Evaluación de la capacidad de remoción de cadmio mediante levaduras (Rhodotorula mucilaginosa y Cryptococcus gastricus), aisladas del volcán Tungurahua.(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2016-06) Jácome Gavilánez, Marco Antonio; Dugarte Jiménez, Nahir YerelyThe removal capacity of cadmium by Rhodotorula mucilaginosa, Cryptococcus gastricus and Saccharomyces cerevisiae was determined by the colorimetric dithizone method. Cadmium removal by the yeasts was studied a pH of 6 and in an incubator orbital at a temperature of 27 ° C, and at concentrations of 34240 mg/l of cadmium, the removal rates were higher than 95 %, showing a high death rate of the yeasts in the removal process. In addition, the process of removing cadmium was tested at concentrations of 2140 mg/l, maintaining the conditions described above. In this case Rhodotorula mucilaginous showed a removal capacity of 100 % of heavy metal while C. gastricus and S. cerevisiae had a maximum capacity of 98,5 % and 99,5 % respectively.