Carrera Ingeniería Bioquímica
Permanent URI for this collectionhttp://repositorio.uta.edu.ec/handle/123456789/809
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Item Efecto in vitro de Valeriana rígida y Valeriana Decussata en la apoptosis tardía en células cancerígenas(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2021-01) Lizano Guzmán, César Paúl; Vargas López, José Homero; Carrero Castillo, Yenddy NayghitCancer represents a public health problem worldwide, especially breast cancer, which occupies the first place of incidence in the country, followed by thyroid cancer. Ecuador has a very biodiverse territory with an extensive variety of medicinal plants that offer a possible therapeutic potential; however, there are few studies that validate these properties. Reason enough to contemplate a number of possibilities in the molecular and biochemical study of different types of plants, in particular of the valerian ones. The objective of this project was to evaluate the expression of the p53 gene by means of qRT-PCR of the Valeriana rigid and Decussata extracts in the MCF7 cell line, in addition to late apoptosis. The results showed that the relative expression of the pro-apoptotic gene p53 in relation to the control showed that the treatments; Valerian Decussata root and Decussata leaf stem produce increased expression of the p53 gene. On the contrary, for Valerian Rigida root and Rigida leaf stem showed a lower expression than that of the control. Regarding the late apoptosis tests using APO / bdru Tunel for control, MCF7 cancer cells were evidenced, with fragmented nuclei. For the treatment with Valeriana Decussata Root, cell fragments and other DNA fragments were observed that may show the end of the apoptotic pathway, as Valeriana Decussata leaf stem only one apoptotic cell was observed, while for rigid Valerian leaf stem and root no evidence of late apoptosis was observed.Item Capacidad proapoptótica in vitro de Valeriana rígida y Valeriana decussata en células cancerígenas(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2021-01) Williams Ibarra, Jeniffer Andrea; Carrero Castillo, Yenddy NayghitCancer represents a public health problem level world, with high incidence and mortality rates in developed and undeveloped countries. Currently there are different therapeutic treatments to eliminate or prevent the proliferation of cancer cells, however, the most are invasive, and they have side effects. Today there are natural therapeutic alternatives with the aim of develop more efficient and less invasive treatments to fight cancer. Faced with this problem, the research done it is based on in vitro evaluation of the proapoptotic and cytotoxic capacity of valerian extracts on the breast cancer cell line. Cell viability assays (MTT), immunohistochemistry assays were performed by fluorescence microscopy and quantitative reverse transcription PCR for gene expression of proteins. The results obtained showed the decrease in cell proliferation and variability in the gene expression of apoptotic proteins in breast cancer cells after being treated with optimal doses of Valeriana rigid and Valeriana decussata extracts.Item Evaluación de la capacidad citotóxica y proapoptótica del látex de Brosimum utile (Sande) sobre células HeLa como posible alternativa terapéutica para el tratamiento del cáncer de cuello uterino(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2018-08) Acosta Ganán, Michael Andrés; Carrero Castillo, Yendy Nayghit; López Hernández, Orestes Darío