Carrera Ingeniería Bioquímica

Permanent URI for this collectionhttp://repositorio.uta.edu.ec/handle/123456789/809

Browse

Subject: search.filters.subject.CANALES DE RIEGO

Search Results

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Determinación molecular de la biodiversidad de levaduras asociada al Canal de riego Latacunga - Salcedo - Ambato (CRLSA).
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2016-05) Hidalgo Escobar, Edith Paola; Pérez Betancourt, Yunys
    The Latacunga-Salcedo-Ambato irrigation channel (LSAIC) is an important environment for agriculture located in the central region of the country. About 24000 ha of crops are irrigated with this water. However, the channel receives large flows of pollutants that result from the neighboring industrial activity. To determine the diversity of yeast within this habitat, seven sampling points were selected along the 36 km comprising the channel. From the samples of water taken, 13 yeast species belonging to 10 genera were isolated. Phylogenetic analysis showed a heterogeneous population, where 77% of the population belongs to the phylum Ascomycota compared with 23% corresponding to the phylum Basidiomycota. Moreover, the molecular diversity found in LSAIC was compared with other polluted waters environments (River Santiago Argentina, River Danube Bratislava, River Doce Brazil) with similar physicochemical characteristics. It was determined that most of the yeast population was different.
  • No Thumbnail Available
    Item
    Análisis de la resistencia a estrés por metales pesados de levaduras aisladas del canal de riego Latacunga - Salcedo - Ambato.
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2016-03) Quinaluisa Calvopiña, Nancy Elizabeth; Serrano Bueno, Gloria
    The Latacunga – Salcedo – Ambato irrigation channel (LSAIC) supplies important agricultural areas in the Cotopaxi and Tungurahua provinces, in which products are distributed all throughout Ecuador. The proximity of the Tungurahua and Cotopaxi volcanoes jointed to the discharges of the canal for tanneries, textile industries, wastewaters, slaughterhouses, factories, hospitals, and agricultural products, make this ecosystem susceptible space for contamination by heavy metals. The resistance analysis of native yeasts of this channel to heavy metal stress using a preliminary concentration of 0.2 and 2 mM showed a high resistance to Zn (100% of the species) and Pb (76.92%). On the contrary, Cd was the most tolerated heavy metal, since only 53.85% of fungal species showed growth in the presence of 0.2mM of the metal. Five of yeast strains showed tolerance to all heavy metals under study, Pichia kluyveri, Candida oleophila, Pichia fermentans, Cystofilobasidium infirmominiatum and Metschnikowia pulcherrima. Although, only seven yeast strains were resistant to 0.2mM of Cd (Cryptococcus faraegula and Rhodotorula mucilaginosa), two of them resisted high concentrations of 2 mM of this metal in a second drop growth experiment. The analysis of contamination by Cd, in the LSAIC, showed that this extreme ecosystem acts as perfect niche for organisms characterized by their ability to adapt limiting conditions.